Diurnal variation in lipoprotein-associated phospholipase A(2) (Lp-PLA(2)).

Author(s): Kaminsky LA, Ozemek C

Publication: Clin Biochem, 2012, Vol. 45, Page 700-2

PubMed ID: 22465237 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of time of collection, patient age, and gender on Lp-PLA2 mass and activity in serum. Serum was stored at -80 degrees C until analysis.

   Summary of Findings:

   Lp-PLA2 mass was significantly lower in specimens collected at 2:00 am compared to those collected at 2:00 pm or 6:00 pm (p=0.047 and p=0.03, respectively), but no other significant differences in Lp-PLA2 mass were noted between collection time points. Lp-PLA2 mass was not significantly affected by patient age or gender. Lp-PLA2 activity was significantly lower in specimens collected at 2:00 am than those collected at 6:00 am (p=0.04), but no other significant differences in Lp-PLA2 activity were noted between collection time points. Lp-PLA2 activity was significantly lower among specimens collected from women compared to those collected from men (p=0.01), but patient age had no effect on Lp-PLA2 activity.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	ELISA
   Protein	Colorimetric assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Time of biospecimen collection	2:00 am 6:00 am 10:00 am 2:00 pm 6:00 pm 10:00 pm
   Preaquisition	Patient age	<40 years >40 years
   Preaquisition	Patient gender	Female Male

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