Reference intervals for saliva analytes collected by a standardized method in a physically active population.

Author(s): Nunes LA, Brenzikofer R, Macedo DV

Publication: Clin Biochem, 2011, Vol. 44, Page 1440-4

PubMed ID: 21986592 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of time of collection and collecting serum rather than saliva on TAS, UA, TP and SAA activity in physically active individuals. Saliva was collected immediately before blood collection. Morning specimens were collected after a 12 h fast and afternoon specimens were collected after a 4 fast. Specimens were stored at 4 degrees C for less than 30 minutes prior to analysis.

   Summary of Findings:

   Saliva volume, SAA and TP were lower in saliva collected in the morning than in specimens collected in the afternoon (p=0.032, p<0.0001 and p<0.0001, respectively), but UA and TAS were not affected by time of collection. A weak correlation was observed between saliva and serum levels of UA (r=0.341) and TP (r=0.212). SAA activity was moderately correlated with serum levels in the morning (r=0.5825), but was only weakly correlated in the afternoon (r=0.2681). There was no correlation between TAS in serum and saliva.

   Biospecimens

   • Bodily Fluid - Saliva
   • Bodily Fluid - Serum

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Enzyme assay
   Protein	Spectrophotometry
   Small molecule	Spectrophotometry
   Small molecule	Clinical chemistry/auto analyzer
   Cell count/volume	Macroscopic observation

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Biospecimen location	Saliva Serum
   Biospecimen Acquisition	Time of biospecimen collection	6:30 AM 4:00 PM

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