Quantitative examination of mechanophysical tumor cell enrichment in fine-needle aspiration specimens.
Author(s): Ernst LM, Rimm DL
Publication: Cancer, 2002, Vol. 96, Page 275-9
PubMed ID: 12378594 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to compare the percentage of malignant cells observed in papanicolaou stained cytolyte fixed FNAs with that observed in H&E stained FFPE surgical breast carcinoma specimens. Two pathologists determined the number of malignant and benign cells in each of 5 20x images per specimen.
Summary of Findings:
The FNA slides and the FFPE sections contained 305-1192 (mean 572.2) and 3071-6049 (mean 4525) cells, respectively. In the four cases of infiltrating ductal carcinoma, there were more malignant cells than benign cells, but this was not true for the single medullary carcinoma specimen. When the specimen was obtained by FNA, there were 1.9-12.7 (mean 6.7) malignant cells for every benign cell. In contrast, surgical specimens only had 0.6-3.6 malignant cells for every benign cell. The total percentages of malignant cells were 65.7-92.7% in FNA specimens and 36.9-77.9% in surgical specimens (p=0.0009).
Biospecimens
Preservative Types
- Formalin
- Other Preservative
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Cell count/volume H-and-E microscopy Cell count/volume Light microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Cytolyte
Formalin (buffered)
Preaquisition Diagnosis/ patient condition Infiltrating ductal carcinoma
Medullary carcinoma
Biospecimen Acquisition Biospecimen location Breast cells
Breast tissue
Biospecimen Acquisition Method of tissue acquisition Surgical resection
Fine needle aspiration