Cryopreservation of whole blood samples collected in the field for a large epidemiologic study.

Author(s): Stevens VL, Patel AV, Feigelson HS, Rodriguez C, Thun MJ, Calle EE

Publication: Cancer Epidemiol Biomarkers Prev, 2007, Vol. 16, Page 2160-3

PubMed ID: 17932366 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of transport time and temperature and duration of storage at -80 degrees C prior to liquid nitrogen storage on lymphocyte recovery, viability, and transformability by EBV of blood specimens.

   Summary of Findings:

   Transport temperatures ranging from 3 to 13 degrees C and time to processing ranging between 4 and 14 hours did not significantly influence lymphocyte recovery, viability or transformability with EBV. Storage at -80 degrees C for between 63 and 256 hours prior to long-term storage at -150 degrees C also showed no effects. Samples showed viability between 87% and 91%. Between 32% and 100% of lymphocytes were recovered and were able to be transformed by EBV within 8 to 17 days.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Light microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	Sodium heparin
   Storage	Specimen transport duration/condition	37-55 degrees C 4.9-13.9 h
   Storage	Storage temperature	-80 degrees C -156 degrees C
   Storage	Storage duration	63-256 h
   Storage	Between site transportation method	Courier

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