Successful downstream application of the Paxgene Blood RNA system from small blood samples in paediatric patients for quantitative PCR analysis.
Author(s): Carrol ED, Salway F, Pepper SD, Saunders E, Mankhambo LA, Ollier WE, Hart CA, Day P
Publication: BMC Immunol, 2007, Vol. 8, Page 20
PubMed ID: 17850649 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the threshold of blood volume required for successful RNA extraction using the PAXgene Blood RNA extraction system. The ratio of blood to kit reagent was held constant for all specimen volumes assessed.
Summary of Findings:
While RNA quantity was proportional to aliquot volume, RNA integrity was independent of the blood volume used for extraction. Real-time qRT-PCR analysis of GAPDH and seven reference genes using RNA extracted from 0.3 ml of whole blood as template was successful.
Biospecimens
Preservative Types
- PAXgene
Diagnoses:
- Normal
- Pneumonia/Respiratory Infection
Platform:
Analyte Technology Platform RNA Automated electrophoresis/Bioanalyzer RNA Real-time qRT-PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Aliquot size/volume 0.3 ml
1.0 ml
2.5 ml