Effects of storage time and leucocyte burden of packed and buffy-coat depleted red blood cell units on red cell storage lesion.

Author(s): Kamel N, Goubran F, Ramsis N, Ahmed AS

Publication: Blood Transfus, 2010, Vol. 8, Page 260-6

PubMed ID: 20967167 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storing pRBCs or RBCs after buffy coat removal for up to 28 days on CD47 staining, the percentages of PS and annexin V positive cells, hemolysis rate, and potassium levels. Blood was collected into double and triple bags containing citrate-phosphate-dextrose-adenine (CPDA). Plasma only or plasma and buffy coat were separated into an empty bag via centrifugation. Storage took place at 4 degrees C under standard blood banking conditions.

   Summary of Findings:

   CD47 fluorescence decreased and the percentages of annexin V positive cells and hemolysis increased during storage of pRBCs or RBC concentrates without buffy coat (p<0.05, all), but the hemolysis rate remained below 0.8% regardless of storage duration. The authors report potassium increased with storage of pRBCs and RBC concentrates without buffy coat. CD47 was lower in pRBCs than RBCs without buffy coat, regardless of storage duration (p<0.05). There was a moderate negative correlation between white blood cell count and CD47 expression (p=0.520, p<0.05). On day 1, pRBCs without buffy coat exhibited less annexin V staining than pRBCs with buffy coat (p<0.05), but there were no differences between groups on day 14 or 28. Removal of buffy coat had no effect on the hemolysis rates at any time-point.

   Biospecimens

   • Bodily Fluid - Blood
   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry
   Cell count/volume	Hematology/ auto analyzer
   Protein	Hematology/ auto analyzer
   Protein	Spectrophotometry
   Electrolyte/Metal	Ion selective electrode

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	1 day 14 days 28 days
   Biospecimen Aliquots and Components	Blood and blood products	Red blood cells Red blood cells without buffy coat

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