Stability of coagulation proteins in frozen plasma.
Author(s): Woodhams B, Girardot O, Blanco MJ, Colesse G, Gourmelin Y
Publication: Blood Coagul Fibrinolysis, 2001, Vol. 12, Page 229-36
PubMed ID: 11460005 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of frozen storage temperature, duration, and aliquot size on the stability of coagulation proteins in normal plasma specimens.
Summary of Findings:
Variations of less than 5% were observed for at least 6 months in all analytes stored at -74 degrees C and for 3 months if storage was at -24 degrees C. In specimens stored at -74 degrees C, PT, thrombin time (TT), fibrinogen, factor V, factor X, protein C and von Willebrand factor (vWF) varied by less than 5% for at least a year and aPTT, factor II, factor VII, factor IX, factor XII, antithrombin, plasminogen and D-dimer varied by less than 5% after the full two years. In specimens stored at -24 degrees C, variation of fibrinogen, protein C and vWF was less than 5% for at least a year and antithrombin, plasminogen and D-dimer variation remained under 5% for the full two years. No effect of freezing at -74 degrees C prior to storage at -24 degrees C versus freezing and storage at -24 degrees C on analyte stability was found. Freezing in one ml versus five ml tubes increased stability of PT, aPTT and factor V when stored at -24 degrees C, but had no effect on other analytes or when specimens were stored at -74 degrees C.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Morphology Hematology/ auto analyzer Protein Hematology/ auto analyzer Peptide Hematology/ auto analyzer Glycoprotein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature -24 degrees C
-74 degrees C
Storage Storage duration 0 weeks
2 weeks
1 month
2 months
3 months
4 months
5 months
6 months
7 months
8 months
9 months
10 months
11 months
12 months
13 months
14 months
15 months
16 months
17 months
18 months
19 months
20 months
21 months
22 months
23 months
24 months
Biospecimen Aliquots and Components Aliquot size/volume 1 ml
5 ml
Biospecimen Preservation Cooling or freezing method/ rate Freezing at -74 degrees C
Freezing at -24 degrees C