Quantitative evaluation of protein expression as a function of tissue microarray core diameter: is a large (1.5 mm) core better than a small (0.6 mm) core?
Author(s): Anagnostou VK, Lowery FJ, Syrigos KN, Cagle PT, Rimm DL
Publication: Arch Pathol Lab Med, 2010, Vol. 134, Page 613-9
PubMed ID: 20367312 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of tissue microarray core size on IHC AQUA scores for biomarkers in breast cancer, lung cancer and melanoma specimens. A randomly chosen single field of 0.6 mM was compared to a composite of several fields representing a 1 mM or 1.5 mM spot.
Summary of Findings:
Correlations of 0.94 and 0.92 were observed between HER2 and ER AQUA scores for 0.6 mM fields and 1.5 mM spots on a breast cancer tissue microarray. AQUA scores for mammalian target of rapamycin (mTOR)and p70S6K in 0.6 mM fields and 1.5 mM spots of lung cancer had correlations of 0.96 and 0.98, respectively, and AQUA scores for epidermal growth factor receptor (EGFR) and signal transducer and activator of transcription 3 (STAT3) in lung cancer had correlations of 0.98 and 0.86 for 0.6 mM fields and 1.0 mM spots. When a 0.6 mM field was compared to a 1 mM spot of melanoma, correlations of 0.92 and 0.90 were observed for mTOR and p70S6K. There were no significant differences between AQUA scores of different sections of the same array.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
- Neoplastic - Melanoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Protein Tissue microarray Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Biospecimen location Lung cancer
Breast cancer
Melanoma
Biospecimen Aliquots and Components Aliquot size/volume 0.6 mm field
1.0 mm spot
1.5 mm spot
Immunohistochemistry Specific Targeted peptide/protein HER-2/neu
ER alpha
EGFR
STAT3
mTOR
p70S6K