Pneumatic tube system induced haemolysis: assessing sample type susceptibility to haemolysis.

Author(s): Sodi R, Darn SM, Stott A

Publication: Ann Clin Biochem, 2004, Vol. 41, Page 237-40

PubMed ID: 15117440 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collection container and PTS transport on hemolysis in blood specimens.

   Summary of Findings:

   The median HI of serum collected in plain serum tubes and transported by PTS was 37, which exceeded the hemolysis threshold of 31 and was significantly higher than the median HI of 4 observed in serum collected in plain serum tubes and transported by porter (p<0.0001). The HI of specimens transported by PTS in 13 mm serum-gel (10), and K-EDTA(6) were significantly higher than when transported by courier (6, p=0.0367, and 2, p=0.0001, respectively). The HI of specimens collected in fluoride-EDTA tubes and transported by PTS were significantly lower than when hand-delivered (6 versus 13, p=0.0021). Significantly higher HI were observed when specimens were collected in 13 mm serum gel tubes rather than 16 mm serum gel tubes and transported by PTS (p=0.046) or hand-delivered (p=0.013).

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Within hospital transportation method	Hand-delivered Pneumatic tube system
   Biospecimen Acquisition	Type of collection container/solution	Plain serum tube 16 mm serum-gel tube 13 mm serum-gel tube
   Biospecimen Acquisition	Anticoagulant	Fluoride-EDTA Lithium heparin Potassium EDTA None

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