Effects of venous stasis on serum concentrations of thyroid hormones and their binding proteins.

Author(s): Hopton MR, Harrop JS

Publication: Ann Clin Biochem, 1982, Vol. 19, Page 134-5

PubMed ID: 6803654 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of tourniquet application on thyroid hormones and thyroid binding protein levels in serum. The authors do not describe preparation of serum from blood specimens and whether the serum was stored prior to analysis. Blood was collected from one arm without venous occlusion and from the other arm with either 3 minutes of tourniquet application or 2 minutes of tourniquet application along with fist clenching.

   Summary of Findings:

   T4, T3, TBG, PA, and albumin were all significantly higher in blood collected after 2 or 3 minutes of tourniquet application compared with levels measured in serum from blood collected with no tourniquet application. Free T4 was significantly higher in serum when specimens were collected with 3 minutes of tourniquet application but not 2 minutes, compared to no tourniquet application.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Peptide	Radioimmunoassay
   Peptide	Immunoassay
   Protein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Requested patient to clench fist during phlebotomy Requested that patient not clench fist during phlebotomy Tourniquet times compared

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