High-resolution analysis of paraffin-embedded and formalin-fixed prostate tumors using comparative genomic hybridization to genomic microarrays.
Author(s): Paris PL, Albertson DG, Alers JC, Andaya A, Carroll P, Fridlyand J, Jain AN, Kamkar S, Kowbel D, Krijtenburg PJ, Pinkel D, Schröder FH, Vissers KJ, Watson VJ, Wildhagen MF, Collins C, Van Dekken H
Publication: Am J Pathol, 2003, Vol. 162, Page 763-70
PubMed ID: 12598311 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to evaluate the utility of archival FFPE prostate cancer tissue for mapping copy number abnormalities by aCGH.
Summary of Findings:
The authors conclude that direct random prime labeling of DNA extracted from archival FFPE specimens for aCGH produced a signal to noise ratio sufficient without the need for a probe amplification step. Results of aCGH were 90% concordant with those obtained by CGH. Further, several copy number abnormalities were observed by aCGH but not CGH, suggesting a higher resolution. Copy number losses, when observed, were confirmed by the quantitative microsatellite analysis (QuMA) approach which is based off the real-time quantitative PCR platform.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform DNA Array CGH DNA Real-time qPCR DNA CGH Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Array CGH Specific Technology platform Array CGH
CGH
Quantitative microsatellite analysis