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Use of the BD vacutainer rapid serum tube reduces false-positive results for selected beckman coulter Unicel DxI immunoassays.

Author(s): Strathmann FG, Ka MM, Rainey PM, Baird GS

Publication: Am J Clin Pathol, 2011, Vol. 136, Page 325-9

PubMed ID: 21757607 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collecting blood in RST rather than PST, and repeat centrifugation on the measurement of 27 clinical chemistry analytes.

   Summary of Findings:

   When specimens collected in RST and PST were compared, values for sodium, potassium, chloride, total carbon dioxide, anion gap, total calcium, glucose, blood urea nitrogen, creatinine, total protein, albumin, phosphorus, magnesium, total bilirubin, direct bilirubin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, amylase, pancreatic amylase, lipase, ethanol, and acetaminophen correlated well (r2>0.95), but clinically significant differences in TnI, CK-MB, hCG and TSH were sometimes seen during the initial analysis. Recentrifugation generally did not affect results for discrepant specimens collected in RST, but in one specimen with an initially high hCG level, repeat centrifugation resulted in a normal result. In contrast, after recentrifugation of specimens collected in PST, lower concentrations of TnI, CK-MB, hCG and TSH were often seen which were more similar to those of specimens collected in RST. In conclusion, collection of blood into RST decreases the number of false positive results for TnI, CK-MB, hCG and TSH compared to collection in PST.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Cardiovascular Disease
   • Not specified

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Gas	Clinical chemistry/auto analyzer
   Glycoprotein	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Vacutainer rapid serum tube Lithium heparin plasma separator tube
   Biospecimen Aliquots and Components	Centrifugation	Different number of centrifugation steps compared
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum

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