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Study Details

PubMed ID:36052213605221
Madisen L, Hoar DI, Holroyd CD, Crisp M, Hodes ME
DNA banking: the effects of storage of blood and isolated DNA on the integrity of DNA.
Am J Med Genet, 1987, Vol. 27, Page 379-90
Review Paper?No
Study Purpose
The purpose of this study was to determine the effects of delayed processing due to transport and storage of whole blood at 3 temperatures, packed cells at 2 temperatures, leukocyte lysates at 3 temperatures, and isolated DNA at 8 different temperatures on the DNA yield and integrity. After experimental storage, some leukocyte lysates were stored for an additional 5 days at 25 degrees C.
Specimen
Fluid Blood
None
None (Fresh)
Platform
DNA
DNA
Electrophoresis
Southern blot
Experimental Factors
Classification Factor Value(s)
Storage Between site transportation method
Mailed
Not transported
Storage Storage temperature
4 degrees C
23 degrees C
25 degrees C
37 degrees C
45 degrees C
55 degrees C
65 degrees C
-70 degrees C
-20 degrees C
Storage Storage duration
0 h
21 h
3 days
4 days
5 days
7 days
8 days
2 weeks
3 weeks
4 weeks
5 weeks
6 weeks
7 weeks
8 weeks
9 weeks
10 weeks
26 weeks
Storage Storage conditions
DNA over chloroform
Leukocyte lysate
Whole blood
Packed cells
Biospecimen Preservation Type of fixation/preservation
Frozen
None (fresh)
Refrigeration
Summary of Findings
Generally, storage of whole blood at -70 degrees C for up to 8 weeks did not affect DNA yield or integrity, but 1 of the 6 specimens consistently had a much lower yield (average 2 versus 25 ug/mL) in specimens stored frozen (2, 4, or 8 weeks) than in the corresponding fresh specimen; however, when packed cells or whole blood were stored at 4 or 23 degrees C, there was a linear decrease in DNA yield observable at 3 days (first time point), regardless of storage conditions. Further, the mean DNA yield was lower in 18 blood specimens shipped at ambient temperatures from France to Indianapolis (6 days) than in the specimens collected from the next 18 patients and processed in house within 24 h. Storage of cell lysates for 2 weeks (shortest duration examined) at 65 degrees C resulted in severe degradation of the DNA, but DNA from lysates stored at 4 or 37 degrees C for 8 weeks remained intact, and storage for an additional 5 days at 25 degrees C did not result in DNA degradation. There was no effect of storage of DNA for up to 6 months, over chloroform, at -70, -20, 4, 25 or 37 degrees C. In contrast, DNA that was stored at 45, 55, or 65 degrees C showed degradation of larger bands after 9 weeks, 6 weeks, and 21 h, respectively.