DNA amplification from formalin-fixed decalcified paraffin-embedded bone marrow trephine specimens: does the duration of storage matter?
Author(s): Talaulikar D, Shadbolt B, McNiven M, Dahlstrom JE
Publication: Pathology, 2008, Vol. 40, Page 702-6
PubMed ID: 18985524 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of storage duration and target fragment length on the amplification of DNA from bone marrow trephine biopsies.
Conclusion of Paper
PCR success in nitric acid decalcified bone marrow trephine biopsies decreased with increasing target fragment length (p<0.0001), but did not correlate with storage duration.
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage duration and target fragment length on the amplification of DNA from bone marrow trephine biopsies.
Summary of Findings:
PCR success in nitric acid decalcified bone marrow trephine biopsies decreased with increasing target fragment length (p<0.0001), but in some cases a long fragment was successfully amplified while a shorter one was not. There was no correlation between storage duration and PCR success.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Lymphoma
Platform:
Analyte Technology Platform DNA PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 4-200 months
PCR Specific Length of gene fragment 96 bp
200 bp
300 bp
400 bp
600 bp
PCR Specific Targeted nucleic acid Thromboxane synthase gene
Recombination activating gene
AF-4 gene
Promyelocytic leukemia zinc-finger gene