Acute Resistance Exercise Modifies Extracellular Vesicle miRNAs Targeting Anabolic Gene Pathways: A Prospective Cohort Study.
Author(s): Conkright WR, Kargl CK, Hubal MJ, Tiede DR, Beckner ME, Sterczala AJ, Krajewski KT, Martin BJ, Flanagan SD, Greeves JP, O'Leary TJ, Wardle SL, Sahu A, Ambrosio F, Nindl BC
Publication: Med Sci Sports Exerc, 2024, Vol. , Page
PubMed ID: 38377006 PubMed Review Paper? No
Purpose of Paper
This paper compared microRNA (miRNA) expression profiles of extracellular vesicles (EVs) obtained from plasma samples obtained before and after resistance exercise. The authors identified signaling pathways that were potentially affected by exercise Based on the known mRNA targets of the differentially expressed miRNAs.
Conclusion of Paper
A total of 34 miRNAs were found to be differentially expressed in plasma EVs from blood that was collected before and immediately after acute heavy resistance exercise test (AHRET). These 34 miRNAs were predicted to target 4,895 mRNAs. Pathways analysis of these mRNAs identified enrichment of 175 pathways, including 12 pathways related to growth and/or metabolism signaling and 8 pathways related to immune/inflammatory signaling. The most significantly affected pathways included p53 signaling, transforming growth factor (TGF)-β signaling, insulin-like growth factor (IGF-1) signaling, interleukin (IL)-8 signaling, and the signal transducer and activator of transcription 3 (STAT3) pathway with 36.7%, 36.5%, 35.2%, 29%, and 31.9%, respectively, of the molecules in the pathways potentially affected by AHRET.
Studies
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Study Purpose
This study compared miRNA expression profiles of EVs obtained from plasma obtained before and after resistance exercise. The authors identified signaling pathways that were potentially affected by exercise based on the known mRNA targets of the differentially expressed miRNAs. Blood was collected from 10 active (exercise ≥30 min, ≥3 times/week) healthy volunteers (5 male and 5 female, 18-36 years of age) into EDTA tubes before and immediately after completion of high resistance exercise (six sets of ten squats with weights that were 75% of the maximum for a single squat). Plasma was obtained by centrifugation of EDTA blood at 1500 g for 15 min at 4°C and stored at -80°C until analysis. Plasma was thawed at 4°C, and extracellular vesicles were isolated by size exclusion chromatography and concentrated using Amicon Ultra-4 Centrifugal Filter Units. RNA was isolated using the Norgen Plasma/Serum Exosome Purification and RNA Isolation Mini Kit and stored at -80°C. RNA was shipped on dry ice and sequenced using an Illumina NextSeq instrument. Differences in expression were identified using a cut-off of a Benjamini-Hochberg false-discovery rate and adjusted P-values <0.05. Pathway analysis was conducted using Ingenuity Pathways Analysis software with a P-value cut-off of <0.1.
Summary of Findings:
A total of 34 miRNAs were found to be differentially expressed by plasma EVs from blood collected pre- versus post-AHRET. These 34 miRNAs were predicted to target 4,895 mRNAs. Pathway analysis of these mRNAs identified enrichment of 175 pathways, including 12 pathways related to growth and/or metabolism signaling and 8 pathways related to immune/inflammatory signaling. The most significantly affected pathways included p53 signaling, TGF-β signaling, IGF-1 signaling, IL-8 signaling, and the STAT3 pathway, with 36.7%, 36.5%, 35.2%, 29%, and 31.9%, respectively, of the molecules in the pathways potentially affected by AHRET.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform RNA Next generation sequencing Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Time of biospecimen collection Pre resistance exercise
Immediately after resistance exercise