Effects of formalin fixation and prolonged block storage on detection of hepatitis C virus RNA in liver tissue.

Author(s): Guerrero RB, Batts KP, Brandhagen DJ, Germer JJ, Perez RG, Persing DH

Publication: Diagn Mol Pathol, 1997, Vol. 6, Page 277-81

PubMed ID: 9458386 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of fixation method and storage of paraffin blocks on the detection of HCV RNA in FFPE liver specimens. Liver specimens were fixed by immersion overnight in formalin, by perfusion with formalin for 3-5 days, or long-term storage in formalin.

   Summary of Findings:

   A 308 bp product of HCV was detected in all specimens fixed by immersion for 24 h and in 85% of specimens fixed by perfusion for 3-5 days, but in none of the specimens stored in formalin long-term. The authors report there was no effect of storage of paraffin blocks on the detection of HCV.

   Biospecimens

   • Tissue - Liver

   Preservative Types

   • Formalin

   Diagnoses:

   • Hepatitis

   Platform:

   Analyte	Technology Platform
   RNA	RT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	2 y 3 y 4 y 5 y 7 y 8 y 9 y 10 y 11 y
   Biospecimen Preservation	Time in fixative	6-24 h 3-5 days Months to 11 years
   Biospecimen Preservation	Method of fixative delivery	Gravity perfusion Immersion
   RT-PCR Specific	Length of gene fragment	308 bp
   RT-PCR Specific	Targeted nucleic acid	Hepatitis C virus

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