The influence of age of template DNA derived from archival tissue on the outcome of the polymerase chain reaction.

Author(s): Hewett PJ, Firgaira F, Morley A

Publication: Aust N Z J Surg, 1994, Vol. 64, Page 558-9

PubMed ID: 8048896 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage of FFPE splenic tissue on DNA degradation as well as amplification success.

   Summary of Findings:

   The authors report that storage duration had no effect on DNA degradation, as determined by electrophoresis. Amplification of the 307 bp fragment of HPRT exon 2 was possible in 15 of 16 specimens that were less than 8 y old, while amplification was possible in 10 of 14 specimens more than 8 y old, however, this difference was not significant. The 1278 bp region of HPRT exon 9 was not amplified in any specimens, regardless of age.

   Biospecimens

   • Tissue - Spleen

   Preservative Types

   • Formalin

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   DNA	Electrophoresis
   DNA	PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	< 8 years > 8 years
   PCR Specific	Length of gene fragment	307 bp 1278 bp
   PCR Specific	Targeted nucleic acid	HPRT

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