DNA extraction from formalin-fixed laryngeal biopsies: Comparison of techniques.

Author(s): Torrente MC, Ríos C, Misad C, Ramírez R, Acuña M, Cifuentes L

Publication: Acta Otolaryngol, 2011, Vol. 131, Page 330-3

PubMed ID: 21142742 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of deparaffinization, extraction method, and amplicon length on successful amplification of HFE from FFPE laryngeal carcinoma specimens.

   Summary of Findings:

   Deparaffinization decreased the amplification success rate when DNA was extracted by proteinase k digestion. When DNA was extracted using the QIAmp FFPE tissue kit, HFE was successfully amplified in all 7 specimens. In contrast, when DNA was extracted using phenol-chloroform, amplification of HFE was successful in only 2 of the 7 specimens, and when DNA was extracted by proteinase k digestion, PCR was successful in 5 of 7 specimens. Amplicon length had no effect on PCR success.

   Biospecimens

   • Tissue - Larynx

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Analyte Extraction and Purification	Deparaffinization	Xylene and ethanol None Alkaline heat
   PCR Specific	Length of gene fragment	208 bp 390 bp
   Analyte Extraction and Purification	Analyte isolation method	Phenol-chloroform QIAmp FFPE tissue Proteinase k digestion

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